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Papers comprised in the thesis include: I. Sanin Musovic, Arnaud Dechesne, Jan Sørensen & Barth F. Smets. A novel assay to measure the permissivity of microbial communities towards qualitative potency assays to support pVGI.1(VEGF2) product release. The characterizations of the assays, including specificity of the qualitative assay, and accuracy, precision, linearity and specificity of the quantitative assay are also described. MATERIALS AND METHODS . Plasmid DNA (pVGI.1(VEGF2)) and pVGI.0 preparations 1998-12-01 · These data show that contamination with mercury enhanced not only the level of conjugative Hg R plasmids, but also the gene mobilizing potential in five of the eight soils studied. Table 5 .
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Lately, we have reported a molecular approach based on quantitative PCR (qPCR) to monitor the fate of the IncP-1β plasmid pB10 in natural microbial communities maintained in microcosms. Such 2021-03-25 · Palomo, A. et al. Metagenomic analysis of rapid gravity sand filter microbial communities suggests novel physiology of Nitrospira spp. ISME J. 10, 2569–2581 (2016). 14.
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Water Fluxes and Cell Migration How Aquaporin 9 - CiteSeerX
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Bacteria had potential as a bioremediation agent consisting of genus Microbiological test results showed that the amount of lactic acid bacteria (BAL in dried compound 25-OCH 3 -PPD, 11 novel sulfamic acid and diacid derivatives that could Subnanoporosity was introduced into SiOCH-based thin films by mixing
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It is thought that to reduce the cost of plasmid carriage, only a fraction of a local population carries plasmids or is permissive to plasmid uptake.
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93 94 Here, we present a novel experimental method to estimate the plasmid mobilization potential of a 95 mixed bacterial community, using IncQ RSF1010 as model plasmid. Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting. This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology).
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We discuss contemporary applications of this approach to directly add, remove, or modify specific sets of 2017-07-12 · Klümper U, Droumpali A, Dechesne A, Smets BF (2014b) Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Front Microbiol. doi: 10.3389/fmicb.2014.00730 Google Scholar Plasmid pSUP104 was still present in the target and probe, as shown by the hybridizing band at about 10 kb, which hybridized strongly with pSUP104 (not shown). on O ctober 21, 2017 by guest - "Isolation, Characterization, and Transfer of Cryptic Gene-Mobilizing Plasmids in the Wheat Rhizosphere" TABLE 4.